sp6 mmessage mmachine in vitro transcription kit am1340 (Thermo Fisher)
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sp6 mmessage mmachine in vitro transcription kit am1340
Sp6 Mmessage Mmachine In Vitro Transcription Kit Am1340, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mmessage+mmachine%C2%AE+sp6+transcription+kit/mmessage+mmachine+t7+transcription+kit/pmc12255298-193-1-8
Average 90 stars, based on 1 article reviews
Sp6 Mmessage Mmachine In Vitro Transcription Kit Am1340, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mmessage+mmachine%C2%AE+sp6+transcription+kit/mmessage+mmachine+t7+transcription+kit/pmc12255298-193-1-8
Average 90 stars, based on 1 article reviews
sp6 mmessage mmachine in vitro transcription kit am1340 - by Bioz Stars,
2026-10
90/100 stars
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Virus:Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia Article Snippet: The pCS2- Stat3 , pCS2- Stat3Y705F and pCS2- Stat3S727A vectors were previously obtained by Peron and collaborators [ ]. mRNAs were in vitro transcribed using the Article Title: Transcriptome remodelling and changes in growth and cardiometabolic phenotype result following Grb10a knockdown in the early life of the zebrafish Article Snippet: Capped RNA was generated using the Article Title: An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome. Article Snippet: Briefly, to produce the SpCas9-FKB and the SaCas9-FRB mRNA for the microinjection, pCS2+ plasmids were linearized with NotI restriction digestion and used as template for in vitro transcription using the Article Title: NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems. Article Snippet: Forty ng of linearized Article Title: Establishing dorsal-ventral patterning in human neural tube organoids with synthetic organizers. Article Snippet: In brief Luo et al. developed a method to generate dorsal-ventral (D-V) patterned neural tube organoids (NTOs) from both zebrafish and human pluripotent stem cells (hPSCs) by engineering opposing BMP and SHH signaling centers.. The human NTOs recapitulate the full complement of 13 neural progenitor subtypes arranged along the D-V axis, with transcriptomic profiling revealing high similarity to the human embryonic spinal cord at Carnegie stage 12. Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein. Article Snippet: Capped RNA transcripts were synthesised by utilizing the SP6 polymerase promoter situated upstream from the CHIKV insert using the mMESSAGE mMACHINE® SP6 transcription kit according to the manufacturer’s instructions ( Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia. Article Snippet: The pCS2-Stat3, pCS2-Stat3Y705F and pCS2-Stat3S727A vectors were previously obtained by Peron and collaborators [43]. mRNAs were in vitro transcribed using the Article Title: Activator-blocker model of transcriptional regulation by pioneer-like factors Article Snippet: Uncropped gel images for Figure S7 Supplementary Table REAGENT or Recombinant:Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia Article Snippet: The pCS2- Stat3 , pCS2- Stat3Y705F and pCS2- Stat3S727A vectors were previously obtained by Peron and collaborators [ ]. mRNAs were in vitro transcribed using the Article Title: Transcriptome remodelling and changes in growth and cardiometabolic phenotype result following Grb10a knockdown in the early life of the zebrafish Article Snippet: Capped RNA was generated using the Article Title: An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome. Article Snippet: Briefly, to produce the SpCas9-FKB and the SaCas9-FRB mRNA for the microinjection, pCS2+ plasmids were linearized with NotI restriction digestion and used as template for in vitro transcription using the Article Title: NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems. Article Snippet: Forty ng of linearized Article Title: Establishing dorsal-ventral patterning in human neural tube organoids with synthetic organizers. Article Snippet: In brief Luo et al. developed a method to generate dorsal-ventral (D-V) patterned neural tube organoids (NTOs) from both zebrafish and human pluripotent stem cells (hPSCs) by engineering opposing BMP and SHH signaling centers.. The human NTOs recapitulate the full complement of 13 neural progenitor subtypes arranged along the D-V axis, with transcriptomic profiling revealing high similarity to the human embryonic spinal cord at Carnegie stage 12. Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein. Article Snippet: Capped RNA transcripts were synthesised by utilizing the SP6 polymerase promoter situated upstream from the CHIKV insert using the mMESSAGE mMACHINE® SP6 transcription kit according to the manufacturer’s instructions ( Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia. Article Snippet: The pCS2-Stat3, pCS2-Stat3Y705F and pCS2-Stat3S727A vectors were previously obtained by Peron and collaborators [43]. mRNAs were in vitro transcribed using the Article Title: Activator-blocker model of transcriptional regulation by pioneer-like factors Article Snippet: Uncropped gel images for Figure S7 Supplementary Table REAGENT or Cloning:Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia Article Snippet: The pCS2- Stat3 , pCS2- Stat3Y705F and pCS2- Stat3S727A vectors were previously obtained by Peron and collaborators [ ]. mRNAs were in vitro transcribed using the Article Title: Transcriptome remodelling and changes in growth and cardiometabolic phenotype result following Grb10a knockdown in the early life of the zebrafish Article Snippet: Capped RNA was generated using the Article Title: An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome. Article Snippet: Briefly, to produce the SpCas9-FKB and the SaCas9-FRB mRNA for the microinjection, pCS2+ plasmids were linearized with NotI restriction digestion and used as template for in vitro transcription using the Article Title: NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems. Article Snippet: Forty ng of linearized Article Title: Establishing dorsal-ventral patterning in human neural tube organoids with synthetic organizers. Article Snippet: In brief Luo et al. developed a method to generate dorsal-ventral (D-V) patterned neural tube organoids (NTOs) from both zebrafish and human pluripotent stem cells (hPSCs) by engineering opposing BMP and SHH signaling centers.. The human NTOs recapitulate the full complement of 13 neural progenitor subtypes arranged along the D-V axis, with transcriptomic profiling revealing high similarity to the human embryonic spinal cord at Carnegie stage 12. Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein. Article Snippet: Capped RNA transcripts were synthesised by utilizing the SP6 polymerase promoter situated upstream from the CHIKV insert using the mMESSAGE mMACHINE® SP6 transcription kit according to the manufacturer’s instructions ( Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia. Article Snippet: The pCS2-Stat3, pCS2-Stat3Y705F and pCS2-Stat3S727A vectors were previously obtained by Peron and collaborators [43]. mRNAs were in vitro transcribed using the Article Title: Activator-blocker model of transcriptional regulation by pioneer-like factors Article Snippet: Uncropped gel images for Figure S7 Supplementary Table REAGENT or Plasmid Preparation:Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia Article Snippet: The pCS2- Stat3 , pCS2- Stat3Y705F and pCS2- Stat3S727A vectors were previously obtained by Peron and collaborators [ ]. mRNAs were in vitro transcribed using the Article Title: Transcriptome remodelling and changes in growth and cardiometabolic phenotype result following Grb10a knockdown in the early life of the zebrafish Article Snippet: Capped RNA was generated using the Article Title: An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome. Article Snippet: Briefly, to produce the SpCas9-FKB and the SaCas9-FRB mRNA for the microinjection, pCS2+ plasmids were linearized with NotI restriction digestion and used as template for in vitro transcription using the Article Title: NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems. Article Snippet: Forty ng of linearized Article Title: Establishing dorsal-ventral patterning in human neural tube organoids with synthetic organizers. Article Snippet: In brief Luo et al. developed a method to generate dorsal-ventral (D-V) patterned neural tube organoids (NTOs) from both zebrafish and human pluripotent stem cells (hPSCs) by engineering opposing BMP and SHH signaling centers.. The human NTOs recapitulate the full complement of 13 neural progenitor subtypes arranged along the D-V axis, with transcriptomic profiling revealing high similarity to the human embryonic spinal cord at Carnegie stage 12. Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein. Article Snippet: Capped RNA transcripts were synthesised by utilizing the SP6 polymerase promoter situated upstream from the CHIKV insert using the mMESSAGE mMACHINE® SP6 transcription kit according to the manufacturer’s instructions ( Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia. Article Snippet: The pCS2-Stat3, pCS2-Stat3Y705F and pCS2-Stat3S727A vectors were previously obtained by Peron and collaborators [43]. mRNAs were in vitro transcribed using the Article Title: Activator-blocker model of transcriptional regulation by pioneer-like factors Article Snippet: Uncropped gel images for Figure S7 Supplementary Table REAGENT or Microinjection:Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia Article Snippet: The pCS2- Stat3 , pCS2- Stat3Y705F and pCS2- Stat3S727A vectors were previously obtained by Peron and collaborators [ ]. mRNAs were in vitro transcribed using the Article Title: Transcriptome remodelling and changes in growth and cardiometabolic phenotype result following Grb10a knockdown in the early life of the zebrafish Article Snippet: Capped RNA was generated using the Article Title: An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome. Article Snippet: Briefly, to produce the SpCas9-FKB and the SaCas9-FRB mRNA for the microinjection, pCS2+ plasmids were linearized with NotI restriction digestion and used as template for in vitro transcription using the Article Title: NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems. Article Snippet: Forty ng of linearized Article Title: Establishing dorsal-ventral patterning in human neural tube organoids with synthetic organizers. Article Snippet: In brief Luo et al. developed a method to generate dorsal-ventral (D-V) patterned neural tube organoids (NTOs) from both zebrafish and human pluripotent stem cells (hPSCs) by engineering opposing BMP and SHH signaling centers.. The human NTOs recapitulate the full complement of 13 neural progenitor subtypes arranged along the D-V axis, with transcriptomic profiling revealing high similarity to the human embryonic spinal cord at Carnegie stage 12. Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein. Article Snippet: Capped RNA transcripts were synthesised by utilizing the SP6 polymerase promoter situated upstream from the CHIKV insert using the mMESSAGE mMACHINE® SP6 transcription kit according to the manufacturer’s instructions ( Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia. Article Snippet: The pCS2-Stat3, pCS2-Stat3Y705F and pCS2-Stat3S727A vectors were previously obtained by Peron and collaborators [43]. mRNAs were in vitro transcribed using the Article Title: Activator-blocker model of transcriptional regulation by pioneer-like factors Article Snippet: Uncropped gel images for Figure S7 Supplementary Table REAGENT or In Vitro:Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia Article Snippet: The pCS2- Stat3 , pCS2- Stat3Y705F and pCS2- Stat3S727A vectors were previously obtained by Peron and collaborators [ ]. mRNAs were in vitro transcribed using the Article Title: Transcriptome remodelling and changes in growth and cardiometabolic phenotype result following Grb10a knockdown in the early life of the zebrafish Article Snippet: Capped RNA was generated using the Article Title: An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome. Article Snippet: Briefly, to produce the SpCas9-FKB and the SaCas9-FRB mRNA for the microinjection, pCS2+ plasmids were linearized with NotI restriction digestion and used as template for in vitro transcription using the Article Title: NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems. Article Snippet: Forty ng of linearized Article Title: Establishing dorsal-ventral patterning in human neural tube organoids with synthetic organizers. Article Snippet: In brief Luo et al. developed a method to generate dorsal-ventral (D-V) patterned neural tube organoids (NTOs) from both zebrafish and human pluripotent stem cells (hPSCs) by engineering opposing BMP and SHH signaling centers.. The human NTOs recapitulate the full complement of 13 neural progenitor subtypes arranged along the D-V axis, with transcriptomic profiling revealing high similarity to the human embryonic spinal cord at Carnegie stage 12. Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein. Article Snippet: Capped RNA transcripts were synthesised by utilizing the SP6 polymerase promoter situated upstream from the CHIKV insert using the mMESSAGE mMACHINE® SP6 transcription kit according to the manufacturer’s instructions ( Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia. Article Snippet: The pCS2-Stat3, pCS2-Stat3Y705F and pCS2-Stat3S727A vectors were previously obtained by Peron and collaborators [43]. mRNAs were in vitro transcribed using the Article Title: Activator-blocker model of transcriptional regulation by pioneer-like factors Article Snippet: Uncropped gel images for Figure S7 Supplementary Table REAGENT or Protease Inhibitor:Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia Article Snippet: The pCS2- Stat3 , pCS2- Stat3Y705F and pCS2- Stat3S727A vectors were previously obtained by Peron and collaborators [ ]. mRNAs were in vitro transcribed using the Article Title: Transcriptome remodelling and changes in growth and cardiometabolic phenotype result following Grb10a knockdown in the early life of the zebrafish Article Snippet: Capped RNA was generated using the Article Title: An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome. Article Snippet: Briefly, to produce the SpCas9-FKB and the SaCas9-FRB mRNA for the microinjection, pCS2+ plasmids were linearized with NotI restriction digestion and used as template for in vitro transcription using the Article Title: NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems. Article Snippet: Forty ng of linearized Article Title: Establishing dorsal-ventral patterning in human neural tube organoids with synthetic organizers. Article Snippet: In brief Luo et al. developed a method to generate dorsal-ventral (D-V) patterned neural tube organoids (NTOs) from both zebrafish and human pluripotent stem cells (hPSCs) by engineering opposing BMP and SHH signaling centers.. The human NTOs recapitulate the full complement of 13 neural progenitor subtypes arranged along the D-V axis, with transcriptomic profiling revealing high similarity to the human embryonic spinal cord at Carnegie stage 12. Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein. Article Snippet: Capped RNA transcripts were synthesised by utilizing the SP6 polymerase promoter situated upstream from the CHIKV insert using the mMESSAGE mMACHINE® SP6 transcription kit according to the manufacturer’s instructions ( Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia. Article Snippet: The pCS2-Stat3, pCS2-Stat3Y705F and pCS2-Stat3S727A vectors were previously obtained by Peron and collaborators [43]. mRNAs were in vitro transcribed using the Article Title: Activator-blocker model of transcriptional regulation by pioneer-like factors Article Snippet: Uncropped gel images for Figure S7 Supplementary Table REAGENT or Multiplex Assay:Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia Article Snippet: The pCS2- Stat3 , pCS2- Stat3Y705F and pCS2- Stat3S727A vectors were previously obtained by Peron and collaborators [ ]. mRNAs were in vitro transcribed using the Article Title: Transcriptome remodelling and changes in growth and cardiometabolic phenotype result following Grb10a knockdown in the early life of the zebrafish Article Snippet: Capped RNA was generated using the Article Title: An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome. Article Snippet: Briefly, to produce the SpCas9-FKB and the SaCas9-FRB mRNA for the microinjection, pCS2+ plasmids were linearized with NotI restriction digestion and used as template for in vitro transcription using the Article Title: NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems. Article Snippet: Forty ng of linearized Article Title: Establishing dorsal-ventral patterning in human neural tube organoids with synthetic organizers. Article Snippet: In brief Luo et al. developed a method to generate dorsal-ventral (D-V) patterned neural tube organoids (NTOs) from both zebrafish and human pluripotent stem cells (hPSCs) by engineering opposing BMP and SHH signaling centers.. The human NTOs recapitulate the full complement of 13 neural progenitor subtypes arranged along the D-V axis, with transcriptomic profiling revealing high similarity to the human embryonic spinal cord at Carnegie stage 12. Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein. Article Snippet: Capped RNA transcripts were synthesised by utilizing the SP6 polymerase promoter situated upstream from the CHIKV insert using the mMESSAGE mMACHINE® SP6 transcription kit according to the manufacturer’s instructions ( Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia. Article Snippet: The pCS2-Stat3, pCS2-Stat3Y705F and pCS2-Stat3S727A vectors were previously obtained by Peron and collaborators [43]. mRNAs were in vitro transcribed using the Article Title: Activator-blocker model of transcriptional regulation by pioneer-like factors Article Snippet: Uncropped gel images for Figure S7 Supplementary Table REAGENT or Picogreen Assay:Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia Article Snippet: The pCS2- Stat3 , pCS2- Stat3Y705F and pCS2- Stat3S727A vectors were previously obtained by Peron and collaborators [ ]. mRNAs were in vitro transcribed using the Article Title: Transcriptome remodelling and changes in growth and cardiometabolic phenotype result following Grb10a knockdown in the early life of the zebrafish Article Snippet: Capped RNA was generated using the Article Title: An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome. Article Snippet: Briefly, to produce the SpCas9-FKB and the SaCas9-FRB mRNA for the microinjection, pCS2+ plasmids were linearized with NotI restriction digestion and used as template for in vitro transcription using the Article Title: NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems. Article Snippet: Forty ng of linearized Article Title: Establishing dorsal-ventral patterning in human neural tube organoids with synthetic organizers. Article Snippet: In brief Luo et al. developed a method to generate dorsal-ventral (D-V) patterned neural tube organoids (NTOs) from both zebrafish and human pluripotent stem cells (hPSCs) by engineering opposing BMP and SHH signaling centers.. The human NTOs recapitulate the full complement of 13 neural progenitor subtypes arranged along the D-V axis, with transcriptomic profiling revealing high similarity to the human embryonic spinal cord at Carnegie stage 12. Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein. Article Snippet: Capped RNA transcripts were synthesised by utilizing the SP6 polymerase promoter situated upstream from the CHIKV insert using the mMESSAGE mMACHINE® SP6 transcription kit according to the manufacturer’s instructions ( Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia. Article Snippet: The pCS2-Stat3, pCS2-Stat3Y705F and pCS2-Stat3S727A vectors were previously obtained by Peron and collaborators [43]. mRNAs were in vitro transcribed using the Article Title: Activator-blocker model of transcriptional regulation by pioneer-like factors Article Snippet: Uncropped gel images for Figure S7 Supplementary Table REAGENT or Chromatin Immunoprecipitation:Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia Article Snippet: The pCS2- Stat3 , pCS2- Stat3Y705F and pCS2- Stat3S727A vectors were previously obtained by Peron and collaborators [ ]. mRNAs were in vitro transcribed using the Article Title: Transcriptome remodelling and changes in growth and cardiometabolic phenotype result following Grb10a knockdown in the early life of the zebrafish Article Snippet: Capped RNA was generated using the Article Title: An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome. Article Snippet: Briefly, to produce the SpCas9-FKB and the SaCas9-FRB mRNA for the microinjection, pCS2+ plasmids were linearized with NotI restriction digestion and used as template for in vitro transcription using the Article Title: NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems. Article Snippet: Forty ng of linearized Article Title: Establishing dorsal-ventral patterning in human neural tube organoids with synthetic organizers. Article Snippet: In brief Luo et al. developed a method to generate dorsal-ventral (D-V) patterned neural tube organoids (NTOs) from both zebrafish and human pluripotent stem cells (hPSCs) by engineering opposing BMP and SHH signaling centers.. The human NTOs recapitulate the full complement of 13 neural progenitor subtypes arranged along the D-V axis, with transcriptomic profiling revealing high similarity to the human embryonic spinal cord at Carnegie stage 12. Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein. Article Snippet: Capped RNA transcripts were synthesised by utilizing the SP6 polymerase promoter situated upstream from the CHIKV insert using the mMESSAGE mMACHINE® SP6 transcription kit according to the manufacturer’s instructions ( Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia. Article Snippet: The pCS2-Stat3, pCS2-Stat3Y705F and pCS2-Stat3S727A vectors were previously obtained by Peron and collaborators [43]. mRNAs were in vitro transcribed using the Article Title: Activator-blocker model of transcriptional regulation by pioneer-like factors Article Snippet: Uncropped gel images for Figure S7 Supplementary Table REAGENT or RNA Sequencing:Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia Article Snippet: The pCS2- Stat3 , pCS2- Stat3Y705F and pCS2- Stat3S727A vectors were previously obtained by Peron and collaborators [ ]. mRNAs were in vitro transcribed using the Article Title: Transcriptome remodelling and changes in growth and cardiometabolic phenotype result following Grb10a knockdown in the early life of the zebrafish Article Snippet: Capped RNA was generated using the Article Title: An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome. Article Snippet: Briefly, to produce the SpCas9-FKB and the SaCas9-FRB mRNA for the microinjection, pCS2+ plasmids were linearized with NotI restriction digestion and used as template for in vitro transcription using the Article Title: NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems. Article Snippet: Forty ng of linearized Article Title: Establishing dorsal-ventral patterning in human neural tube organoids with synthetic organizers. Article Snippet: In brief Luo et al. developed a method to generate dorsal-ventral (D-V) patterned neural tube organoids (NTOs) from both zebrafish and human pluripotent stem cells (hPSCs) by engineering opposing BMP and SHH signaling centers.. The human NTOs recapitulate the full complement of 13 neural progenitor subtypes arranged along the D-V axis, with transcriptomic profiling revealing high similarity to the human embryonic spinal cord at Carnegie stage 12. Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein. Article Snippet: Capped RNA transcripts were synthesised by utilizing the SP6 polymerase promoter situated upstream from the CHIKV insert using the mMESSAGE mMACHINE® SP6 transcription kit according to the manufacturer’s instructions ( Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia. Article Snippet: The pCS2-Stat3, pCS2-Stat3Y705F and pCS2-Stat3S727A vectors were previously obtained by Peron and collaborators [43]. mRNAs were in vitro transcribed using the Article Title: Activator-blocker model of transcriptional regulation by pioneer-like factors Article Snippet: Uncropped gel images for Figure S7 Supplementary Table REAGENT or Binding Assay:Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia Article Snippet: The pCS2- Stat3 , pCS2- Stat3Y705F and pCS2- Stat3S727A vectors were previously obtained by Peron and collaborators [ ]. mRNAs were in vitro transcribed using the Article Title: Transcriptome remodelling and changes in growth and cardiometabolic phenotype result following Grb10a knockdown in the early life of the zebrafish Article Snippet: Capped RNA was generated using the Article Title: An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome. Article Snippet: Briefly, to produce the SpCas9-FKB and the SaCas9-FRB mRNA for the microinjection, pCS2+ plasmids were linearized with NotI restriction digestion and used as template for in vitro transcription using the Article Title: NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems. Article Snippet: Forty ng of linearized Article Title: Establishing dorsal-ventral patterning in human neural tube organoids with synthetic organizers. Article Snippet: In brief Luo et al. developed a method to generate dorsal-ventral (D-V) patterned neural tube organoids (NTOs) from both zebrafish and human pluripotent stem cells (hPSCs) by engineering opposing BMP and SHH signaling centers.. The human NTOs recapitulate the full complement of 13 neural progenitor subtypes arranged along the D-V axis, with transcriptomic profiling revealing high similarity to the human embryonic spinal cord at Carnegie stage 12. Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein. Article Snippet: Capped RNA transcripts were synthesised by utilizing the SP6 polymerase promoter situated upstream from the CHIKV insert using the mMESSAGE mMACHINE® SP6 transcription kit according to the manufacturer’s instructions ( Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia. Article Snippet: The pCS2-Stat3, pCS2-Stat3Y705F and pCS2-Stat3S727A vectors were previously obtained by Peron and collaborators [43]. mRNAs were in vitro transcribed using the Article Title: Activator-blocker model of transcriptional regulation by pioneer-like factors Article Snippet: Uncropped gel images for Figure S7 Supplementary Table REAGENT or Purification:Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia Article Snippet: The pCS2- Stat3 , pCS2- Stat3Y705F and pCS2- Stat3S727A vectors were previously obtained by Peron and collaborators [ ]. mRNAs were in vitro transcribed using the Article Title: Transcriptome remodelling and changes in growth and cardiometabolic phenotype result following Grb10a knockdown in the early life of the zebrafish Article Snippet: Capped RNA was generated using the Article Title: An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome. Article Snippet: Briefly, to produce the SpCas9-FKB and the SaCas9-FRB mRNA for the microinjection, pCS2+ plasmids were linearized with NotI restriction digestion and used as template for in vitro transcription using the Article Title: NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems. Article Snippet: Forty ng of linearized Article Title: Establishing dorsal-ventral patterning in human neural tube organoids with synthetic organizers. Article Snippet: In brief Luo et al. developed a method to generate dorsal-ventral (D-V) patterned neural tube organoids (NTOs) from both zebrafish and human pluripotent stem cells (hPSCs) by engineering opposing BMP and SHH signaling centers.. The human NTOs recapitulate the full complement of 13 neural progenitor subtypes arranged along the D-V axis, with transcriptomic profiling revealing high similarity to the human embryonic spinal cord at Carnegie stage 12. Article Title: Attenuation of Chikungunya Virus by a Single Amino Acid Substitution in the nsP1 Component of a Non-Structural Polyprotein. Article Snippet: Capped RNA transcripts were synthesised by utilizing the SP6 polymerase promoter situated upstream from the CHIKV insert using the mMESSAGE mMACHINE® SP6 transcription kit according to the manufacturer’s instructions ( Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia. Article Snippet: The pCS2-Stat3, pCS2-Stat3Y705F and pCS2-Stat3S727A vectors were previously obtained by Peron and collaborators [43]. mRNAs were in vitro transcribed using the Article Title: Activator-blocker model of transcriptional regulation by pioneer-like factors Article Snippet: Uncropped gel images for Figure S7 Supplementary Table REAGENT or |